Black-bone silky fowl (Gallus gallus domesticus Brisson) muscle was hydrolyzed with Alcalase 2.4 L and papain, and treated by multistage separation. The black-bone silky fowl muscle peptides (BSFP) with low molecular weight obtained was tested for antioxidant and angiotensin I-converting enzyme (ACE) inhibitory activities. BSFP was then separated by reversed-phase high performance liquid chromatography. Five major fractions were collected and their 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging and ACE inhibitory activities were assayed. Fractions 3–5 displaying higher activities were subjected to mass spectrometry to identify the active peptides. A total of 29 peptide sequences were identified, and 11 peptides were synthesized for further ABTS radical scavenging and ACE inhibitory activity analysis. Finally, one potent antioxidative peptide (Leu-Trp-Arg, 3.25 ± 0.11 mmol Trolox equivalents/g sample) and two novel potent ACE inhibitory peptides (Leu-Glu-Arg, IC50=45.62 ± 2.40 μM; Gly-Ala-Gly-Pro, IC50=253.07 ± 6.66 μM) were found. Their activities were approximately 6.9, 14.1 and 2.8 times higher than that of BSFP, respectively.
Food-derived peptides with biological activity: from research to food applications.
Rainer Hartmann, Hans Meisel. Current Opinion in Biotechnology, 2007, 18(2): 163–169.
Many peptides that are released in vitro or in vivo from animal or plant proteins are bioactive and have regulatory functions in humans beyond normal and adequate nutrition. Different health effects have been attributed to food-derived peptides, including antimicrobial properties, blood pressure-lowering (ACE inhibitory) effects, cholesterol-lowering ability, antithrombotic and antioxidant activities, enhancement of mineral absorption and/or bioavailability, cyto- or immunomodulatory effects, and opioid activities. Numerous products are already on the market or under development by food companies that exploit the potential of food-derived bioactive peptides and which ascribe scientifically evidenced health claims to consumption of these functional foods.